The Rat ACV-A (Activin A) ELISA Kit is a specialized assay designed for the quantitative detection of Activin A levels in rat samples. Activin A is a member of the transforming growth factor-beta superfamily and plays a crucial role in various physiological processes, including regulation of reproductive system functions, cell growth, differentiation, and development. This ELISA kit offers high sensitivity and specificity, allowing for precise measurement of Activin A levels in rat biological samples.
Accurate quantification of Activin A is essential for studying its functions and implications in the context of reproductive health, embryonic development, and disease pathogenesis. Manufactured under stringent quality control standards, this kit ensures reliable and reproducible results, making it an excellent choice for research in areas related to endocrinology, reproductive biology, and developmental biology.
Product Name:
Rat ACV-A (Activin A) ELISA Kit
SKU:
AEES00285
Target:
Rat ACV-A (Activin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACV-A. You can calculate the concentration of Rat ACV-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
92-109
94-110
Average (%)
91
100
101
1:4
Range (%)
86-100
81-91
87-99
Average (%)
93
87
94
1:8
Range (%)
90-104
82-96
85-98
Average (%)
97
88
90
1:16
Range (%)
89-102
80-94
85-97
Average (%)
94
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
264.88
701.74
1862.85
242.16
693.64
1687.51
Standard deviation
18.12
37.4
92.21
14.09
41.48
52.65
C V (%)
6.84
5.33
4.95
5.82
5.98
3.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ACV-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ACV-A in samples. No significant cross-reactivity or interference between Rat ACV-A and analogues was observed.
