Rat Akr7a2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit
The Rat AKR7A2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit is a powerful tool for the precise quantification of AKR7A2 levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various experimental settings. AKR7A2 is an important enzyme involved in the detoxification of aflatoxin B1, a potent carcinogen found in contaminated food and feeds. By measuring AKR7A2 levels, researchers can gain insight into the detoxification process and potential health risks associated with aflatoxin exposure.
This ELISA kit is suitable for use with rat serum, plasma, and cell lysate samples, providing versatility for a wide range of research applications. With its reliability and robust performance, the Rat AKR7A2 ELISA Kit is an essential tool for studying aflatoxin metabolism and evaluating potential therapeutic interventions.
Product Name:
Rat Akr7a2 (Aflatoxin B1 aldehyde reductase member 2) ELISA Kit
SKU:
RTFI01364
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-101%
87-103%
85-103%
EDTA Plasma (n = 5)
91-99%
85-99%
84-101%
Heparin Plasma (n = 5)
80-97%
81-99%
85-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-102
97
EDTA Plasma (n = 5)
85-105
94
Heparin Plasma (n = 5)
89-102
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
