The Rat ANG2 (Angiopoietin 2) ELISA Kit is a cutting-edge assay meticulously designed to quantitatively detect Angiopoietin 2 levels in a variety of biological samples collected from rat specimens. Angiopoietin 2 is a key player in angiogenesis and vascular biology, influencing blood vessel development, endothelial cell permeability, and tissue repair processes. Its multifaceted role in vascular homeostasis, endothelial dysfunction, and inflammation makes it a critical target for investigations spanning cardiovascular health, tumor angiogenesis, and inflammatory conditions. This advanced ELISA kit by Assay Genie promises exceptional sensitivity and specificity for accurate and reproducible results. Crafted under rigorous quality control measures, this kit ensures robust performance and ease of use, presenting researchers with a reliable tool to elucidate the intricate functions of Angiopoietin 2 in various physiological and pathological phenomena. Uncover the complexities of Angiopoietin 2 biology with confidence using this high-performance ELISA kit.
Product Name:
Rat ANG2 (Angiopoietin 2) ELISA Kit
SKU:
AEES00331
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
