The Rat Apolipoprotein A1 (ApoA1) ELISA Kit is a specialized assay designed to quantitatively assess the levels of ApoA1, a key apolipoprotein responsible for the structural integrity and metabolism of high-density lipoprotein (HDL), in various biological samples obtained from rat models. ApoA1 is an essential component of HDL particles, playing a critical role in reverse cholesterol transport, lipid metabolism, and cardiovascular health.
This ELISA kit offers researchers a precise and reliable method for measuring ApoA1 levels, providing valuable insights into lipid metabolism, cardiovascular health, and atheroprotective functions associated with ApoA1. By accurately quantifying ApoA1 with exceptional sensitivity and specificity, researchers can investigate the role of ApoA1 in lipid transport, cholesterol metabolism, and cardiovascular diseases in rat models. Manufactured under stringent quality control standards, the Rat ApoA1 ELISA Kit ensures robust performance, accurate results, and ease of use, making it an excellent choice for research studies focusing on lipid metabolism, cardiovascular health, and the cardioprotective functions of ApoA1 in rat models.
Product Name:
Rat ApoA1 (Apolipoprotein A1) ELISA Kit
SKU:
AEES00543
Target:
Rat ApoA1 (Apolipoprotein A1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoA1. You can calculate the concentration of Rat ApoA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
90-102
92-100
Average (%)
90
95
97
1:4
Range (%)
87-100
88-98
94-109
Average (%)
93
92
102
1:8
Range (%)
89-101
96-106
97-107
Average (%)
96
101
102
1:16
Range (%)
95-107
92-104
85-97
Average (%)
103
98
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
92
EDTA plasma (n=5)
94-103
98
Cell culture media (n=5)
99-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.45
115.67
422.16
47.64
103.92
485.26
Standard deviation
2.67
5.56
17.77
2.48
4.88
20.82
C V (%)
5.87
4.81
4.21
5.21
4.7
4.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ApoA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoA1 in samples. No significant cross-reactivity or interference between Rat ApoA1 and analogues was observed.
