The Rat APOB (Apolipoprotein B) CLIA Kit is a specialized assay designed for the accurate quantification of Apolipoprotein B levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Apolipoprotein B is a critical protein involved in lipid metabolism and cholesterol transport, playing a key role in the development of cardiovascular diseases and metabolic disorders.
By accurately measuring Apolipoprotein B levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.Overall, the Rat APOB CLIA Kit is an indispensable tool for studying lipid metabolism, cardiovascular health, and related diseases in rat models, providing researchers with reliable data to advance their scientific investigations.
Product Name:
Rat ApoB (Apolipoprotein B) CLIA Kit
SKU:
RTES00615
Target:
Rat ApoB (Apolipoprotein B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 ng/mL
Detection range:
12.50-800 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ApoB. You can calculate the concentration of Rat ApoB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
87-98
101-117
Average (%)
94
92
110
1:4
Range (%)
89-105
100-114
93-108
Average (%)
97
108
99
1:8
Range (%)
88-104
92-105
88-105
Average (%)
95
99
96
1:16
Range (%)
94-109
93-107
86-99
Average (%)
99
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.99
72.23
335.06
42.28
70.51
322.12
Standard deviation
3.32
5.42
26.37
3.8
5.38
33.47
C V (%)
8.52
7.5
7.87
8.99
7.63
10.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ApoB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ApoB in samples. No significant cross-reactivity or interference between Rat ApoB and analogues was observed.
