Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit
The Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit is a powerful tool for researchers studying apoptosis and cell death mechanisms in rats. This kit allows for the accurate and sensitive detection of BAD levels in rat samples, including serum, plasma, and cell lysates.BAD is a key regulator of apoptosis, playing a critical role in the balance between cell survival and death. Dysregulation of BAD has been implicated in various diseases, including cancer and neurodegenerative disorders.
By utilizing this CLIA kit, researchers can gain valuable insights into the role of BAD in these diseases and potentially identify new therapeutic targets.With its high sensitivity and specificity, the Rat BAD CLIA Kit offers reliable and reproducible results, making it an essential tool for any laboratory conducting research on apoptosis and cell death pathways in rats. Don't miss out on the opportunity to advance your research with this innovative kit from AssayGenie.
Product Name:
Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit
SKU:
RTES00060
Target:
Rat BAD (BCL-2 Associated Death Promoter)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BAD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BAD. You can calculate the concentration of Rat BAD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
85-100
97-110
Average (%)
108
91
105
1:4
Range (%)
95-111
89-105
101-116
Average (%)
102
96
110
1:8
Range (%)
95-110
88-104
90-101
Average (%)
101
95
96
1:16
Range (%)
95-106
85-99
89-101
Average (%)
100
90
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
100-114
108
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.51
68.42
399.64
38.6
65.23
435.19
Standard deviation
4.34
7.61
39.72
3.84
6.64
48.61
C V (%)
10.98
11.12
9.94
9.95
10.18
11.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BAD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed.
