The Rat C4 (Complement Component 4) ELISA Kit is a specialized assay designed to accurately quantify the levels of Complement Component 4 in various biological samples obtained from rat models. Complement Component 4 plays a critical role in the complement system, a fundamental part of the immune response responsible for enhancing immune defenses, eliminating pathogens, and promoting inflammation.
Measurement of C4 levels is essential for evaluating the activation status of the complement pathway, immune responses, and inflammatory processes. By utilizing this ELISA kit, researchers can investigate the dynamics of C4 levels in different biological contexts, shedding light on its involvement in immune regulation, infectious diseases, autoimmune disorders, and inflammatory conditions. Understanding the quantitative levels of Complement Component 4 is crucial for unraveling its contribution to host defense mechanisms and immune-mediated diseases, providing insights into potential therapeutic interventions and disease management strategies. The Rat C4 ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, enabling precise and reliable quantification of C4 levels in rat samples. Manufactured with stringent quality control measures, this kit delivers robust and reproducible performance, making it a valuable tool for research endeavors exploring the complex interplay of the complement system and immune responses in various disease states.
Product Name:
Rat C4 (Complement Component 4) ELISA Kit
SKU:
AEES00363
Target:
Rat C4 (Complement Component 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C4. You can calculate the concentration of Rat C4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
92-106
83-97
Average (%)
92
99
90
1:4
Range (%)
90-105
82-96
88-101
Average (%)
98
88
93
1:8
Range (%)
87-102
81-90
89-102
Average (%)
94
86
94
1:16
Range (%)
91-105
80-93
83-95
Average (%)
97
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.49
19.29
75.42
10.97
17.67
77.38
Standard deviation
0.58
0.92
3.93
0.65
0.84
3.75
C V (%)
5.53
4.77
5.21
5.93
4.75
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed.
