The Rat Caspase-1 (Casp1) CLIA Kit is specifically designed for the quantitative measurement of Caspase-1 levels in rat serum, plasma, and cell lysates. This kit offers high sensitivity and precision, ensuring accurate and consistent results for a variety of research applications.Caspase-1 is a key enzyme involved in inflammatory responses and programmed cell death. Dysregulation of Caspase-1 has been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With easy-to-follow protocols and reliable performance, the Rat Caspase-1 (Casp1) CLIA Kit is an essential tool for researchers investigating the role of Caspase-1 in inflammation, immunity, and disease progression in rat models.
Product Name:
Rat CASP1 (Caspase 1) CLIA Kit
SKU:
RTES00204
Target:
Rat CASP1 (Caspase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP1. You can calculate the concentration of Rat CASP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
94-111
97-110
Average (%)
94
102
103
1:4
Range (%)
86-99
95-106
95-111
Average (%)
91
101
102
1:8
Range (%)
92-102
87-101
98-112
Average (%)
97
94
103
1:16
Range (%)
89-103
84-99
91-106
Average (%)
94
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
96-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.15
106.6
333.82
49.68
113.62
346.6
Standard deviation
5.51
12.47
36.55
5.3
12.04
33.27
C V (%)
10.77
11.7
10.95
10.67
10.6
9.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP1 in samples. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed.
