The Rat CASP1 (Caspase 1) ELISA Kit is a specialized assay designed for the quantitative measurement of Caspase 1 levels in various rat biological samples. Caspase 1 is a crucial protein involved in the inflammatory response and plays a significant role in initiating the inflammatory cascade.
It is a key component of the inflammasome, a multi-protein complex responsible for the processing and activation of pro-inflammatory cytokines like IL-1. Accurate quantification of Caspase 1 using this ELISA kit enables researchers to investigate the role of this protease in inflammatory processes, immune responses, and cell death mechanisms. Dysregulation of Caspase 1 activity has been associated with various inflammatory disorders, autoimmune diseases, and infections, making it an essential target for understanding the pathophysiology of these conditions. The Rat CASP1 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit provides reliable performance and delivers accurate data on Caspase 1 levels in biological samples. It serves as a valuable tool for researchers studying inflammatory pathways and developing potential therapeutic interventions targeting Caspase 1.
Product Name:
Rat CASP1 (Caspase 1) ELISA Kit
SKU:
AEES00396
Target:
Rat CASP1 (Caspase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP1. You can calculate the concentration of Rat CASP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
85-99
98-115
Average (%)
103
92
105
1:4
Range (%)
90-104
79-94
87-101
Average (%)
96
86
93
1:8
Range (%)
87-101
86-100
88-99
Average (%)
93
92
93
1:16
Range (%)
92-104
82-92
85-98
Average (%)
98
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.18
599.81
1601.24
191.62
566.55
1738.77
Standard deviation
13.14
27.35
61.49
10.77
31.78
65.73
C V (%)
6.8
4.56
3.84
5.62
5.61
3.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CASP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP1 in samples. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed.
