The Rat CASR (Calcium Sensing Receptor) CLIA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of CASR levels in rat serum, plasma, and tissue homogenates. This kit provides accurate and reproducible results, making it ideal for a variety of research applications.CASR is a vital protein that plays a key role in calcium homeostasis and signaling, affecting processes such as bone metabolism, hormone secretion, and cell proliferation.
Dysregulation of CASR has been implicated in various diseases, including hyperparathyroidism and kidney stones, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat CASR CLIA Kit offers researchers a powerful tool for investigating the role of CASR in physiological and pathological processes, providing valuable insights into its function and potential therapeutic targets.
Product Name:
Rat CASR (Calcium Sensing Receptor) CLIA Kit
SKU:
RTES00079
Target:
Rat CASR (Calcium Sensing Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASR. You can calculate the concentration of Rat CASR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
91-104
90-107
Average (%)
104
96
98
1:4
Range (%)
101-116
95-110
95-112
Average (%)
109
103
102
1:8
Range (%)
99-112
99-114
96-109
Average (%)
105
107
103
1:16
Range (%)
91-104
95-109
99-112
Average (%)
97
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.33
171.54
885.07
109.51
184.81
823.32
Standard deviation
11.82
14.62
84.61
11.84
20.42
57.71
C V (%)
11.78
8.52
9.56
10.81
11.05
7.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASR in samples. No significant cross-reactivity or interference between Rat CASR and analogues was observed.
