The Rat COL3 (Collagen Type 3) CLIA Kit is specifically designed for the quantitative detection of COL3 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in various research applications.Collagen Type 3 is a key component of the extracellular matrix and is essential for maintaining tissue structure and integrity. Abnormal levels of COL3 have been associated with various diseases, including connective tissue disorders and fibrosis.
By accurately measuring COL3 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.Overall, the Rat COL3 CLIA Kit is a valuable tool for studying collagen metabolism and its implications in various disease states, offering researchers a reliable and efficient method for quantitative analysis.
Product Name:
Rat COL3 (Collagen Type 3) CLIA Kit
SKU:
RTES00138
Target:
Rat COL3 (Collagen Type 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL3. You can calculate the concentration of Rat COL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
100-114
90-101
Average (%)
100
107
95
1:4
Range (%)
92-105
99-114
101-115
Average (%)
99
106
109
1:8
Range (%)
86-97
97-112
102-114
Average (%)
92
102
108
1:16
Range (%)
84-97
92-105
84-98
Average (%)
90
98
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.78
119.83
421.76
57.53
116.67
388.36
Standard deviation
6.33
11.05
39.48
6.48
9.29
34.1
C V (%)
11.99
9.22
9.36
11.26
7.96
8.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL3 in samples. No significant cross-reactivity or interference between Rat COL3 and analogues was observed.
