The Rat CTn I (cTnI/TNNI3) Cardiac Troponin I CLIA Kit (RTES00619) is a highly sensitive and specific assay designed for the accurate detection of cardiac troponin I levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of cardiovascular research applications.Cardiac troponin I is a key biomarker for diagnosing and monitoring heart conditions such as myocardial infarction.
Elevated levels of cTnI in the bloodstream indicate cardiac muscle damage, making it a critical marker for assessing heart health and the effectiveness of treatments.With the Rat CTn I (cTnI/TNNI3) Cardiac Troponin I CLIA Kit, researchers can analyze cTnI levels with high precision, allowing for more thorough investigations into heart disease mechanisms and potential therapeutic strategies.
Product Name:
Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit
SKU:
RTES00619
Target:
Rat cTn-I/TNNI3 (Cardiac Troponin I)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cTn-I/TNNI3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTn-I/TNNI3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTn-I/TNNI3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cTn-I/TNNI3. You can calculate the concentration of Rat cTn-I/TNNI3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
85-96
103-115
Average (%)
94
91
109
1:4
Range (%)
98-114
93-105
98-112
Average (%)
107
99
105
1:8
Range (%)
87-102
102-117
87-98
Average (%)
95
108
93
1:16
Range (%)
98-112
89-103
95-110
Average (%)
105
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.14
127.67
380.9
42.34
115.95
403.72
Standard deviation
4.31
14.45
34.93
5.4
10.41
45.38
C V (%)
9.99
11.32
9.17
12.75
8.98
11.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat cTn-I/TNNI3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Rat cTn-I/TNNI3 and analogues was observed.
