The Rat β-EP (Beta-Endorphin) ELISA Kit is meticulously designed to quantitatively assess the levels of Beta-Endorphin in diverse rat biological samples. Beta-Endorphin, a prominent member of the endogenous opioid peptides, holds significant importance in regulating pain perception and the body's stress response. As a natural opioid with potent analgesic properties, Beta-Endorphin plays a vital role in modulating mood, stress, and emotions. Understanding the dynamics of Beta-Endorphin levels is crucial for unraveling its impact on neurological functions, pain management, and emotional well-being.
This ELISA kit is tailored to deliver exceptional sensitivity and specificity, ensuring precise and reproducible results in research applications. Manufactured under strict quality control standards, the Rat β-EP ELISA Kit offers robust performance, making it a reliable choice for researchers exploring the intricate interplay of Beta-Endorphin in various physiological processes. Delve into the realm of opioid peptides and pain modulation with confidence using this advanced ELISA kit, unlocking insights into Beta-Endorphin's role in neurobiology and behavioral responses.
Product Name:
Rat beta-EP (Beta-Endorphin) ELISA Kit
SKU:
AEES00324
Target:
Rat β-EP (Beta-Endorphin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
98-110
95-105
Average (%)
102
103
100
1:4
Range (%)
88-98
91-103
96-112
Average (%)
93
97
102
1:8
Range (%)
87-101
89-101
98-110
Average (%)
94
94
104
1:16
Range (%)
84-97
87-102
94-109
Average (%)
91
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46
96.3
457.4
49.2
97.7
488.2
Standard deviation
3.2
3.9
16.5
2.7
4.2
15.1
C V (%)
6.96
4.05
3.61
5.49
4.3
3.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
