Rat EPCR (Endothelial Protein C Receptor) CLIA Kit
The Rat EPCR (Endothelial Protein C Receptor) CLIA Kit is a high-quality assay kit specifically designed for the accurate measurement of EPCR levels in rat samples. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for various research applications.EPCR is a critical protein involved in the regulation of blood coagulation and inflammation, making it a key biomarker for studying thrombotic disorders, sepsis, and other related conditions.
By accurately quantifying EPCR levels in rat samples, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat EPCR CLIA Kit from Assaygenie is a valuable tool for researchers looking to study the role of EPCR in disease pathophysiology and develop novel treatment strategies.
Product Name:
Rat EPCR (Endothelial Protein C Receptor) CLIA Kit
SKU:
RTES00516
Target:
Rat EPCR (Endothelial Protein C Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EPCR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EPCR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EPCR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EPCR. You can calculate the concentration of Rat EPCR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
99-115
89-103
Average (%)
108
106
94
1:4
Range (%)
87-103
101-119
93-107
Average (%)
94
109
98
1:8
Range (%)
95-107
94-109
97-112
Average (%)
100
100
103
1:16
Range (%)
89-99
94-112
101-118
Average (%)
94
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.59
222.4
767.87
93.4
212.55
700.92
Standard deviation
10.67
25.4
73.95
11.11
17.56
61.61
C V (%)
11.16
11.42
9.63
11.9
8.26
8.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EPCR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EPCR in samples. No significant cross-reactivity or interference between Rat EPCR and analogues was observed.
