The Rat ET-1 (Endothelin-1) CLIA Kit is a reliable and accurate tool for measuring levels of endothelin-1 in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and precise results for a variety of research experiments.Endothelin-1 is a key molecule involved in regulating blood vessel constriction and is known to play a critical role in cardiovascular health. Abnormal levels of endothelin-1 have been linked to conditions such as hypertension, heart failure, and pulmonary diseases, highlighting its importance as a biomarker for studying and understanding these diseases.
The Rat ET-1 CLIA Kit provides researchers with a valuable tool for investigating the role of endothelin-1 in various physiological processes and disease states, ultimately contributing to the development of novel therapeutic strategies.
Product Name:
Rat ET-1 (Endothelin 1) CLIA Kit
SKU:
RTES00100
Target:
Rat ET-1 (Endothelin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ET-1. You can calculate the concentration of Rat ET-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
97-109
91-105
Average (%)
90
103
98
1:4
Range (%)
102-116
97-112
97-113
Average (%)
108
103
105
1:8
Range (%)
91-106
91-108
86-100
Average (%)
98
98
92
1:16
Range (%)
84-100
93-109
90-102
Average (%)
91
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
105
EDTA plasma (n=5)
99-117
107
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.41
18.46
88.02
9.96
16.74
88.9
Standard deviation
1.02
2.09
9.09
1.28
1.51
9.64
C V (%)
10.84
11.32
10.33
12.85
9.02
10.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ET-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ET-1 in samples. No significant cross-reactivity or interference between Rat ET-1 and analogues was observed.
