The Rat ET-1 (Endothelin 1) ELISA Kit is a highly specialized assay designed for the quantitative detection of Endothelin 1 (ET-1) levels in rat samples.
ET-1 is a potent vasoconstrictor peptide produced in endothelial cells and has essential physiological roles in regulating vascular tone, blood pressure, and kidney function. This ELISA kit provides researchers with a precise method to quantify ET-1 concentrations, enabling investigations into cardiovascular diseases, hypertension, and endothelial dysfunction. Accurate measurement of ET-1 levels using this ELISA kit is crucial for understanding its intricate involvement in vascular physiology, cardiovascular disorders, and renal function. The quantitative data obtained from this assay can shed light on the mechanisms underlying vascular pathophysiology, offering valuable insights into the role of ET-1 in health and disease. Researchers studying cardiovascular conditions can benefit from the detailed analysis of ET-1 levels provided by this kit. Assay Genie's Rat ET-1 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, this kit ensures robust performance and ease of use, making it a reliable choice for cardiovascular research and investigations into the regulatory actions of ET-1 in vascular and renal systems.
Product Name:
Rat ET-1 (Endothelin 1) ELISA Kit
SKU:
AEES00512
Target:
Rat ET-1 (Endothelin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ET-1. You can calculate the concentration of Rat ET-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-106
85-96
87-98
Average (%)
101
93
91
1:4
Range (%)
100-110
90-101
98-107
Average (%)
103
97
103
1:8
Range (%)
96-109
98-108
86-96
Average (%)
104
105
92
1:16
Range (%)
100-108
93-103
99-111
Average (%)
104
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
101
EDTA plasma (n=5)
97-104
101
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.14
5.93
24.08
2.31
5.77
23.01
Standard deviation
0.11
0.27
1.08
0.14
0.29
1.14
C V (%)
5.14
4.55
4.49
6.06
5.03
4.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ET-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ET-1 in samples. No significant cross-reactivity or interference between Rat ET-1 and analogues was observed.
