The Rat FGF23 (Fibroblast Growth Factor 23) ELISA Kit is a specialized assay designed for the quantitative detection of FGF23 levels in various biological samples obtained from rat models. FGF23 is a key regulator of phosphate and vitamin D metabolism, playing a crucial role in maintaining mineral homeostasis and bone health. This ELISA kit provides researchers with a precise and reliable method to quantify FGF23 levels, allowing for a comprehensive understanding of its involvement in bone mineralization, renal function, and mineral metabolism.
By accurately measuring FGF23 with exceptional sensitivity and specificity, researchers can advance their knowledge of FGF23's role in various physiological processes and pathological conditions, including chronic kidney disease and genetic disorders affecting mineral metabolism. Manufactured under stringent quality control standards, the Rat FGF23 ELISA Kit ensures robust performance, accurate results, and ease of use, making it an invaluable tool for investigations into the complex interplay of FGF23 in physiological and pathological pathways in rat models.
Product Name:
Rat FGF23 (Fibroblast Growth Factor 23) ELISA Kit
SKU:
AEES00545
Target:
Rat FGF23 (Fibroblast Growth Factor 23)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FGF23. You can calculate the concentration of Rat FGF23 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
94-104
89-101
Average (%)
92
98
96
1:4
Range (%)
88-100
88-101
98-109
Average (%)
92
94
103
1:8
Range (%)
102-112
94-109
101-109
Average (%)
105
101
104
1:16
Range (%)
85-99
86-101
91-104
Average (%)
91
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
102
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
89-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.98
446.2
1851.75
192.4
482.24
1710.53
Standard deviation
10.92
20.57
98.7
10.04
21.89
82.79
C V (%)
5.72
4.61
5.33
5.22
4.54
4.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FGF23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed.
