The Rat FN (Fibronectin) CLIA Kit is a powerful tool for measuring levels of fibronectin in rat biological samples such as serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results for various research applications.Fibronectin is a key extracellular matrix protein that plays a crucial role in cell adhesion, migration, and tissue remodeling processes. Dysregulation of fibronectin expression has been linked to various diseases, including cancer, fibrosis, and inflammatory conditions.
Therefore, studying fibronectin levels can provide valuable insights into disease progression and therapeutic strategies.This Rat FN CLIA Kit offers a convenient and efficient way to quantify fibronectin levels in rat samples, allowing researchers to explore the role of fibronectin in various physiological and pathological processes. Get reliable data to advance your research with this high-quality assay kit from AssayGenie.
Product Name:
Rat FN (Fibronectin) CLIA Kit
SKU:
RTES00355
Target:
Rat FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FN. You can calculate the concentration of Rat FN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
88-100
90-106
Average (%)
100
95
97
1:4
Range (%)
95-110
100-116
98-111
Average (%)
100
108
105
1:8
Range (%)
84-98
97-113
96-108
Average (%)
91
103
101
1:16
Range (%)
100-115
104-117
91-107
Average (%)
108
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
102-119
108
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
1.75
8.62
1.18
1.65
9.26
Standard deviation
0.13
0.17
0.55
0.1
0.19
1.03
C V (%)
11.93
9.71
6.38
8.47
11.52
11.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FN in samples. No significant cross-reactivity or interference between Rat FN and analogues was observed.
