The Rat FN (Fibronectin) ELISA Kit is a specialized assay designed for the precise quantitative measurement of Fibronectin levels in various rat biological samples. Fibronectin is a key glycoprotein that plays a vital role in cell adhesion, migration, growth, and differentiation.
Its involvement in the extracellular matrix and cellular processes makes it a critical player in tissue development, wound healing, and various physiological functions. This ELISA kit, developed by Assay Genie, is equipped with high sensitivity and specificity to ensure accurate and reproducible results. Manufactured under strict quality standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers studying the role of Fibronectin in rat models and its implications in cellular functions and tissue homeostasis.
Product Name:
Rat FN (Fibronectin) ELISA Kit
SKU:
AEES00463
Target:
Rat FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FN. You can calculate the concentration of Rat FN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-101
93-104
Average (%)
105
96
98
1:4
Range (%)
87-101
84-97
85-99
Average (%)
94
89
91
1:8
Range (%)
89-103
79-91
87-101
Average (%)
95
85
93
1:16
Range (%)
90-107
79-93
87-100
Average (%)
98
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.69
11.07
26.98
3.41
11.13
27.73
Standard deviation
0.22
0.57
0.83
0.22
0.53
1.32
C V (%)
5.96
5.15
3.08
6.45
4.76
4.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FN in samples. No significant cross-reactivity or interference between Rat FN and analogues was observed.
