Rat FRS3 (Fibroblast Growth Factor Receptor Substrate 3) CLIA Kit
The Rat FRS3 (Fibroblast Growth Factor Receptor Substrate 3) CLIA Kit is a reliable and accurate assay designed for the detection of FRS3 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FRS3 is a critical protein in cellular signaling pathways, particularly in the regulation of cell growth, differentiation, and survival.
Dysregulation of FRS3 has been implicated in various diseases such as cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and potentially developing therapeutics.Overall, the Rat FRS3 CLIA Kit provides researchers with a powerful tool for investigating the role of FRS3 in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Rat FRS3 (Fibroblast Growth Factor Receptor Substrate 3) CLIA Kit
SKU:
RTES00210
Target:
Rat FRS3 (Fibroblast Growth Factor Receptor Substrate 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FRS3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FRS3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FRS3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FRS3. You can calculate the concentration of Rat FRS3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
85-99
90-104
Average (%)
95
91
97
1:4
Range (%)
101-115
91-103
90-104
Average (%)
107
97
96
1:8
Range (%)
91-107
89-104
98-111
Average (%)
97
96
104
1:16
Range (%)
86-102
101-116
97-109
Average (%)
93
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
91-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.39
282.77
857.56
97.36
291.57
835.42
Standard deviation
8.74
30.54
71.18
7.99
20.5
80.28
C V (%)
8.45
10.8
8.3
8.21
7.03
9.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FRS3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FRS3 in samples. No significant cross-reactivity or interference between Rat FRS3 and analogues was observed.
