The Rat FSH (Follicle Stimulating Hormone) CLIA Kit is a powerful tool for researchers looking to accurately measure FSH levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FSH is a key hormone that plays a critical role in the reproductive system, specifically in the development of ovarian follicles and the regulation of egg maturation.
Imbalances in FSH levels can have significant impacts on fertility and overall reproductive health, making this kit an invaluable resource for studying reproductive disorders and potential therapeutic interventions.With its reliable performance and ease of use, the Rat FSH CLIA Kit is an essential for any researcher seeking to delve into the complexities of reproductive biology and hormone regulation in rats.
Product Name:
Rat FSH (Follicle Stimulating Hormone) CLIA Kit
SKU:
RTES00216
Target:
Rat FSH (Follicle Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FSH. You can calculate the concentration of Rat FSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-117
100-116
98-112
Average (%)
110
106
104
1:4
Range (%)
99-112
91-103
89-102
Average (%)
105
97
96
1:8
Range (%)
99-113
91-106
97-112
Average (%)
106
97
104
1:16
Range (%)
100-118
92-106
99-113
Average (%)
108
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
96-113
103
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
5.51
17.6
2.09
6.05
19.2
Standard deviation
0.19
0.61
1.93
0.2
0.45
1.68
C V (%)
9.95
11.07
10.97
9.57
7.44
8.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FSH in samples. No significant cross-reactivity or interference between Rat FSH and analogues was observed.
