The Rat FSH (Follicle Stimulating Hormone) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of FSH levels in rat biological samples.
Follicle-stimulating hormone (FSH) is a crucial glycoprotein hormone produced by the anterior pituitary gland and plays a pivotal role in the regulation of reproductive processes, specifically in the maintenance of fertility and gametogenesis. Accurate detection and quantification of FSH levels are essential for studying reproductive physiology, fertility issues, and endocrine system disorders. Monitoring changes in FSH concentrations can provide valuable insights into ovarian function and reproductive health. The Rat FSH ELISA Kit enables researchers to investigate the physiological role of FSH and its impact on fertility and reproductive health in rat models. Assay Genie's Rat FSH ELISA Kit offers high sensitivity and specificity, ensuring precise and reproducible results. Manufactured under rigorous quality control standards, this kit guarantees optimal performance and reliability, making it an ideal choice for researchers and scientists involved in reproductive biology and endocrinology research.
Product Name:
Rat FSH (Follicle Stimulating Hormone) ELISA Kit
SKU:
AEES00402
Target:
Rat FSH (Follicle Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FSH. You can calculate the concentration of Rat FSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
87-103
95-109
Average (%)
101
94
102
1:4
Range (%)
101-117
81-94
97-110
Average (%)
107
87
104
1:8
Range (%)
100-115
86-98
100-110
Average (%)
106
91
105
1:16
Range (%)
98-113
82-95
91-106
Average (%)
105
87
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
88-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.5
22.0
75.7
10.9
21.5
75.2
Standard deviation
0.7
1.1
3.3
0.7
1.3
3.8
C V (%)
6.67
5.0
4.36
6.42
6.05
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FSH in samples. No significant cross-reactivity or interference between Rat FSH and analogues was observed.
