Rat G-CSF (Granulocyte Colony Stimulating Factor) CLIA Kit
The Rat G-CSF (Granulocyte Colony Stimulating Factor) CLIA Kit is specifically designed for the accurate measurement of G-CSF levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for research applications.G-CSF is a vital cytokine involved in the regulation of granulocyte production and function, playing a crucial role in the immune response to infection and inflammation.
Dysregulation of G-CSF levels has been implicated in various diseases, making it a key biomarker for studying immune-related disorders and developing potential therapeutic interventions.Overall, the Rat G-CSF CLIA Kit is an essential tool for researchers investigating the role of G-CSF in health and disease, offering reliable and accurate measurements for insightful data analysis.
Product Name:
Rat G-CSF (Granulocyte Colony Stimulating Factor) CLIA Kit
SKU:
RTES00261
Target:
Rat G-CSF (Granulocyte Colony Stimulating Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat G-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat G-CSF. You can calculate the concentration of Rat G-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
99-115
91-107
Average (%)
99
107
99
1:4
Range (%)
89-103
101-120
96-114
Average (%)
94
109
104
1:8
Range (%)
93-109
91-103
86-97
Average (%)
101
97
92
1:16
Range (%)
87-99
91-104
89-100
Average (%)
93
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.52
66.11
200.17
25.03
64.74
196.61
Standard deviation
3.06
6.99
14.31
2.15
6.49
13.8
C V (%)
11.12
10.57
7.15
8.59
10.02
7.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat G-CSF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat G-CSF in samples. No significant cross-reactivity or interference between Rat G-CSF and analogues was observed.
