The Rat GAL3 (Galectin 3) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of Galectin 3 levels in rat samples. Galectin 3 is a multifunctional protein involved in various biological processes, including inflammation, cell proliferation, differentiation, and apoptosis. Its role in modulating immune responses, cell adhesion, and signaling pathways underscores its significance in diverse physiological and pathological conditions. Accurate detection and quantification of Galectin 3 levels are crucial for investigating its involvement in disease mechanisms, particularly in conditions related to inflammation, fibrosis, cancer, and cardiovascular diseases.
The Rat GAL3 ELISA Kit provides researchers with a reliable tool to explore the role of Galectin 3 in cellular processes and pathological conditions in rat models. With exceptional sensitivity and specificity, the Rat GAL3 ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research in the fields of immunology, inflammation, and disease pathology.
Product Name:
Rat GAL3 (Galectin 3) ELISA Kit
SKU:
AEES00403
Target:
Rat GAL3 (Galectin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GAL3. You can calculate the concentration of Rat GAL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
85-98
97-111
Average (%)
101
92
103
1:4
Range (%)
87-103
84-98
89-101
Average (%)
94
90
94
1:8
Range (%)
90-104
85-100
85-100
Average (%)
95
92
92
1:16
Range (%)
88-98
81-96
81-95
Average (%)
93
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.98
274.43
937.93
105.57
286.75
924.15
Standard deviation
5.58
12.71
37.7
5.5
16.03
37.71
C V (%)
5.7
4.63
4.02
5.21
5.59
4.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GAL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL3 in samples. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed.
