The Rat GH (Growth Hormone) ELISA Kit is expertly crafted to facilitate the precise and quantitative analysis of growth hormone levels in various biological samples collected from rats. Growth hormone is a pivotal endocrine regulator essential for promoting growth, regulating metabolism, and influencing various physiological processes. Through accurate measurement of growth hormone concentrations, this ELISA kit enables in-depth exploration of growth-related mechanisms and physiological responses in rat models. Accurate quantification of growth hormone levels is crucial for understanding its role in growth and development, as well as its involvement in metabolic activities and overall health. By employing this specialized ELISA assay, researchers can gain valuable insights into the dynamics of growth hormone concentrations under different experimental conditions, providing a deeper understanding of growth regulation and its implications in health and disease. The Rat GH ELISA Kit by Assay Genie delivers exceptional sensitivity and specificity, ensuring precise, reproducible, and dependable results. Rigorously manufactured under stringent quality controls, this kit ensures robust performance and user-friendly operation, making it an excellent choice for researchers investigating growth hormone biology and its impact on diverse physiological processes.
Product Name:
Rat GH (Growth Hormone) ELISA Kit
SKU:
AEES00519
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
