Rat Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit
The Rat Glutathione Peroxidase 3 (GPX3) Plasma ELISA Kit is a reliable and sensitive assay designed for the accurate detection of GPX3 levels in rat plasma samples. This kit provides high specificity and precision, ensuring consistent and reproducible results for a variety of research applications.GPX3 is an important antioxidant enzyme that plays a key role in protecting cells from oxidative damage and maintaining cellular homeostasis. It is involved in various physiological processes and is implicated in conditions such as cancer, cardiovascular diseases, and inflammatory disorders.
The measurement of GPX3 levels can provide valuable insights into the pathophysiology of these diseases and help in the development of potential therapeutic strategies.With its ability to accurately quantify GPX3 levels in rat plasma, the Rat GPX3 Plasma ELISA Kit is an essential tool for researchers studying oxidative stress, antioxidant defense mechanisms, and related disease mechanisms in rats. Trust in the reliability and precision of this ELISA kit to advance your research and uncover new discoveries in the field of antioxidant biology.
Product Name:
Rat Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
