Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
The Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit is a specialized assay designed for the quantitative detection of GM-CSF levels in rat biological samples.
GM-CSF is a crucial cytokine that plays a pivotal role in the regulation of hematopoiesis and immune responses, particularly in stimulating the growth and differentiation of granulocytes and macrophages. This ELISA kit offers exceptional sensitivity and specificity, ensuring the accurate measurement of GM-CSF concentrations in rat samples. GM-CSF is an essential factor in leukocyte production and immune function, making it a key target for research in immunology, hematopoiesis, and inflammatory conditions. Manufactured under rigorous quality control standards, this ELISA kit provides robust performance, reliable results, and ease of use, making it an excellent choice for researchers studying GM-CSF and its impact on immune regulation and hematopoiesis in rats.
Product Name:
Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
SKU:
AEES00291
Target:
Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GM-CSF. You can calculate the concentration of Rat GM-CSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
85-98
96-108
Average (%)
106
91
102
1:4
Range (%)
93-109
84-100
85-98
Average (%)
99
91
90
1:8
Range (%)
88-103
84-95
87-99
Average (%)
95
89
92
1:16
Range (%)
95-106
83-97
86-97
Average (%)
100
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.74
130.81
345.85
50.91
121.73
371.14
Standard deviation
2.61
5.57
11.27
3.22
4.89
13.14
C V (%)
5.25
4.26
3.26
6.32
4.02
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GM-CSF in samples. No significant cross-reactivity or interference between Rat GM-CSF and analogues was observed.
