Rat GRO b Recombinant Protein (RPPB1149)
- SKU:
- RPPB1149
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P30348
- Research Area:
- Chemokines
Description
| Product Name: | Rat GRO b Recombinant Protein |
| Product Code: | RPPB1149 |
| Size: | 20µg |
| Species: | Rat |
| Target: | GRO b |
| Synonyms: | Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine (C-X-C motif) ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta, CINC-3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized GRO-beta Rat in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized GRO-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 99.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN |
| Biological Activity: | The biological activity was determined by its ability to chemoattract total human neutrophils using a concentration range of 1.0-10.0 ng/ml, corresponding to a specific activity of 100,000-1,000,000 units/mg. |
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
GRO-Beta Rat Recombinant also called Rat MIP-2 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7923 Dalton. The GRO-b is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis Cellular Component: cytosol; extracellular space Molecular Function:chemokine activity; CXCR chemokine receptor binding Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte chemotaxis; neutrophil chemotaxis; regulation of cell proliferation; response to amphetamine; response to estradiol stimulus; response to gamma radiation; response to glucocorticoid stimulus; response to heat; response to molecule of bacterial origin; wound healing |
| NCBI Summary: | chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006] |
| UniProt Code: | P30348 |
| NCBI GenInfo Identifier: | 266536 |
| NCBI Gene ID: | 114105 |
| NCBI Accession: | P30348.1 |
| UniProt Secondary Accession: | P30348,Q5WA60, |
| UniProt Related Accession: | P30348 |
| Molecular Weight: | 10,783 Da |
| NCBI Full Name: | C-X-C motif chemokine 2 |
| NCBI Synonym Full Names: | C-X-C motif chemokine ligand 2 |
| NCBI Official Symbol: | Cxcl2�� |
| NCBI Official Synonym Symbols: | Mip-2; Scyb2�� |
| NCBI Protein Information: | C-X-C motif chemokine 2 |
| UniProt Protein Name: | C-X-C motif chemokine 2 |
| UniProt Synonym Protein Names: | Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2 |
| Protein Family: | C-X-C motif chemokine |
| UniProt Gene Name: | Cxcl2�� |
| UniProt Entry Name: | CXCL2_RAT |
