Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit
The Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit is a specialized assay designed for the precise and quantitative detection of ICAM-1 levels in various rat biological samples. ICAM-1 is a crucial cell surface glycoprotein involved in mediating cell adhesion and immune responses. Its expression and regulation are of significant interest in the context of inflammation, immune cell interactions, and various pathological conditions. Accurate quantification of ICAM-1 levels using this ELISA kit is essential for elucidating its role in cell adhesion processes, inflammatory responses, and disease pathogenesis. Researchers can harness the data obtained from this assay to gain insights into the mechanisms underlying immune cell communication, inflammatory disorders, and the development of potential therapeutic interventions targeting ICAM-1 pathways. With its exceptional sensitivity and specificity, the Rat ICAM-1/CD54 ELISA Kit from Assay Genie ensures reliable and reproducible results. Manufactured according to stringent quality control standards, this kit offers robust performance and ease of use, making it a valuable tool for research studies focused on understanding the function and implications of ICAM-1 in a variety of biological contexts.
Product Name:
Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit
SKU:
AEES00515
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
