The Rat IFN Beta (Interferon Beta) CLIA Kit is specifically designed for the quantitative detection of IFN Beta levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.IFN Beta is a key cytokine involved in the immune response, playing a crucial role in antiviral defense and modulating inflammatory processes. Dysregulation of IFN Beta levels has been implicated in various autoimmune diseases, making it an important biomarker for studying these conditions and developing targeted therapies.
Overall, the Rat IFN Beta CLIA Kit is a valuable tool for researchers looking to study the role of IFN Beta in immune response and inflammatory diseases in rat models. Its high performance and ease of use make it an ideal choice for laboratories conducting cutting-edge research in this field.
Product Name:
Rat IFN- beta (Interferon Beta) CLIA Kit
SKU:
RTES00328
Target:
Rat IFN- beta (Interferon Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IFN-β. You can calculate the concentration of Rat IFN-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
94-109
84-99
Average (%)
98
101
91
1:4
Range (%)
100-118
97-113
86-100
Average (%)
109
105
92
1:8
Range (%)
88-100
93-105
94-110
Average (%)
93
99
102
1:16
Range (%)
102-115
85-95
100-118
Average (%)
109
90
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
88-103
96
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.71
72.67
178.1
27.56
70.91
178.27
Standard deviation
2.48
7.08
11.49
2.79
8.1
15.03
C V (%)
9.28
9.74
6.45
10.12
11.42
8.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFN-β in samples. No significant cross-reactivity or interference between Rat IFN-β and analogues was observed.
