The Rat IFN-Gamma (Interferon-Gamma) CLIA Kit is specifically designed for the quantitative detection of IFN-Gamma levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research studies.IFN-Gamma is a key cytokine involved in the immune response, playing a critical role in regulation of inflammation, host defense against pathogens, and immune cell activation. Dysregulation of IFN-Gamma has been linked to various diseases such as autoimmune disorders, infectious diseases, and cancer, making it a valuable biomarker for investigating these conditions and potential therapeutic targets.
With easy-to-follow protocols and a simple assay procedure, the Rat IFN-Gamma CLIA Kit is a valuable tool for researchers studying immune responses and inflammatory processes in rat models. Trust Assay Genie for high-quality kits to advance your research efforts.
Product Name:
Rat IFN-gamma (Interferon Gamma) CLIA Kit
SKU:
RTES00009
Target:
Rat IFN-gamma (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
100-112
90-105
Average (%)
93
106
98
1:4
Range (%)
85-97
90-102
89-101
Average (%)
91
95
94
1:8
Range (%)
86-99
101-113
101-116
Average (%)
93
107
109
1:16
Range (%)
93-105
86-97
98-113
Average (%)
100
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.29
111.11
379.65
39.6
105.39
391.62
Standard deviation
3.66
12.86
29.88
4.94
10.75
30.62
C V (%)
8.86
11.57
7.87
12.47
10.2
7.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
