The Rat IFN-β (Interferon Beta) ELISA Kit is a specialized assay crafted for the precise quantitative measurement of Interferon Beta levels in diverse biological samples. Interferon Beta is a key cytokine that plays a pivotal role in the regulation of the immune response, particularly in viral infections and autoimmune diseases. Understanding the levels of Interferon Beta can provide critical insights into the body's immune reaction dynamics and its contribution to various immune-related conditions. Accurate quantification of IFN-β is essential for unraveling its role in immune function, disease pathogenesis, and the development of targeted therapeutic interventions. With exceptional sensitivity and specificity, Assay Genie's IFN-β ELISA Kit offers reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance, making it a valuable tool for cutting-edge research in immunology and infectious diseases.
Product Name:
Rat IFN-beta (Interferon Beta) ELISA Kit
SKU:
AEES00448
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
