The Rat IgA (Immunoglobulin A) CLIA Kit is a powerful tool for measuring IgA levels in rat serum, plasma, and other biological samples. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for a variety of research applications.IgA is an important antibody that plays a key role in the immune system by targeting and neutralizing pathogens at mucosal surfaces. Monitoring IgA levels can provide valuable insights into the immune response and help researchers study a wide range of conditions, from infections to autoimmune diseases.
Whether you are studying the effects of a specific treatment on IgA levels or investigating the immune response to a particular disease, the Rat IgA CLIA Kit is an essential tool for your research needs. Trust AssayGenie's expertise and innovation to enhance your research efforts and drive new discoveries in the field of immunology.
Product Name:
Rat IgA (Immunoglobulin A) CLIA Kit
SKU:
RTES00303
Target:
Rat IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IgA. You can calculate the concentration of Rat IgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
87-98
91-103
Average (%)
92
92
97
1:4
Range (%)
88-99
93-104
95-109
Average (%)
94
98
102
1:8
Range (%)
90-106
104-119
89-101
Average (%)
97
110
96
1:16
Range (%)
102-119
94-108
88-103
Average (%)
108
100
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
87-103
95
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.99
17.83
89.35
9.23
16.33
94.15
Standard deviation
1.16
1.5
6.68
0.95
1.36
8.25
C V (%)
11.61
8.41
7.48
10.29
8.33
8.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgA in samples. No significant cross-reactivity or interference between Rat IgA and analogues was observed.
