Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit
The Rat Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) ELISA Kit is a specialized assay designed to quantitatively detect and measure the levels of IGFBP-3 in various biological samples from rats. IGFBP-3 plays a crucial role in modulating the activity and bioavailability of insulin-like growth factors, particularly IGF-1, which are essential for cell growth and proliferation. This ELISA kit enables researchers to accurately determine IGFBP-3 concentrations, providing valuable insights into the regulation of IGF signaling pathways, cell growth control, and overall metabolic functions.
By offering exceptional sensitivity and specificity, this kit allows for precise and reliable measurement of IGFBP-3 levels, crucial for understanding its involvement in growth regulation, development processes, and potential implications in various physiological and pathological conditions. The Rat IGFBP-3 ELISA Kit by Assay Genie delivers robust and reproducible results, ensuring data accuracy and consistency in research studies focused on IGF-related mechanisms and growth factor signaling pathways. Manufactured under stringent quality control standards, this kit offers high performance and user-friendly protocols, making it an ideal choice for researchers investigating the complex interactions of IGFBP-3 in growth and development processes.
Product Name:
Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit
SKU:
AEES00531
Target:
Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGFBP-3. You can calculate the concentration of Rat IGFBP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-102
97-107
98-109
Average (%)
99
102
103
1:4
Range (%)
93-106
86-97
96-107
Average (%)
101
92
99
1:8
Range (%)
94-105
98-106
96-109
Average (%)
97
103
101
1:16
Range (%)
100-111
86-99
95-108
Average (%)
103
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
93
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
98-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
80.92
232.99
905.55
84.62
202.15
876.18
Standard deviation
4.69
10.97
37.4
4.9
9.99
38.11
C V (%)
5.8
4.71
4.13
5.79
4.94
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IGFBP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP-3 in samples. No significant cross-reactivity or interference between Rat IGFBP-3 and analogues was observed.
