The Rat IgG (Immunoglobulin G) ELISA Kit is meticulously designed to quantitatively analyze the levels of Immunoglobulin G (IgG) in a diverse range of biological samples. Immunoglobulin G is one of the major antibody classes present in the immune system, playing a key role in the defense against pathogens and infections. IgG antibodies are crucial for long-term immune responses, including providing protection against viruses, bacteria, and other invaders.
Accurate measurement of IgG levels is vital for understanding immune responses, diagnosing infectious diseases, and monitoring antibody-related conditions. With the Rat IgG ELISA Kit from Assay Genie, researchers can achieve precise and reliable results with exceptional sensitivity and specificity. This kit is manufactured under stringent quality control standards to ensure robust performance, making it an excellent choice for immunological research and immune system studies.
Product Name:
Rat IgG (Immunoglobulin G) ELISA Kit
SKU:
AEES00440
Target:
Rat IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgG. You can calculate the concentration of Rat IgG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-105
93-107
96-114
Average (%)
100
98
104
1:4
Range (%)
94-110
84-96
91-106
Average (%)
100
91
99
1:8
Range (%)
93-111
84-98
98-113
Average (%)
101
90
104
1:16
Range (%)
99-113
87-102
95-111
Average (%)
104
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11
29.1
92.1
11.1
29.7
86.1
Standard deviation
0.6
1.7
4.1
0.7
1.7
4.4
C V (%)
5.45
5.84
4.45
6.31
5.72
5.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgG in samples. No significant cross-reactivity or interference between Rat IgG and analogues was observed.
