The Rat IgG2a (Immunoglobulin G2a) ELISA Kit is specifically designed for the accurate detection of rat IgG2a levels in serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a variety of research applications.Immunoglobulin G2a is an important antibody isotype with diverse functions in the immune response, including opsonization, neutralization of pathogens, and activation of complement. Monitoring IgG2a levels can provide valuable insights into the immune status and response in various disease models and experimental settings.
Whether studying autoimmune diseases, infectious diseases, or immunological responses, the Rat IgG2a ELISA Kit is a valuable tool for researchers looking to accurately measure and analyze IgG2a levels in rat samples. Trust in the reliability and performance of this kit to advance your research and uncover new insights into immune function and disease pathology.
Product Name:
Rat IgG2a (Immunoglobulin G2a) ELISA Kit
SKU:
RTES01043
Target:
Rat IgG2a (Immunoglobulin G2a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG2a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgG2a. You can calculate the concentration of Rat IgG2a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
91-104
90-103
Average (%)
102
96
95
1:4
Range (%)
93-107
88-99
88-98
Average (%)
100
93
93
1:8
Range (%)
91-106
86-100
90-103
Average (%)
97
93
95
1:16
Range (%)
88-102
86-100
88-99
Average (%)
95
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.98
44.64
178.96
18.66
42.88
172.46
Standard deviation
1
1.88
6.91
1.28
1.74
7.23
C V (%)
5.01
4.21
3.86
6.86
4.06
4.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IgG2a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgG2a in samples. No significant cross-reactivity or interference between Rat IgG2a and analogues was observed.
