The Rat IL-1α ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-1 alpha (IL-1α) levels in various rat biological samples.
IL-1α is a crucial pro-inflammatory cytokine that plays a pivotal role in a wide range of cellular activities, including immune responses, cell proliferation, differentiation, and apoptosis. Its involvement in inflammatory processes and immune regulation makes accurate measurement of IL-1α essential for understanding its role in inflammatory diseases, autoimmune conditions, infections, and other immune-related disorders in rats. The IL-1α ELISA Kit offered by Assay Genie features exceptional sensitivity and specificity, providing reliable and reproducible results for researchers. This kit is manufactured under rigorous quality control standards to ensure robust performance and ease of use, making it a valuable tool for research applications focused on investigating the functions and implications of IL-1α in biological systems.
Product Name:
Rat IL-1alpha ELISA Kit
SKU:
AEES00293
Target:
Rat IL-1α
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1α. You can calculate the concentration of Rat IL-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
90-103
88-102
Average (%)
98
97
96
1:4
Range (%)
96-113
84-97
93-108
Average (%)
104
91
99
1:8
Range (%)
101-116
82-91
95-108
Average (%)
107
87
102
1:16
Range (%)
98-116
82-92
97-111
Average (%)
106
88
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.6
159.9
473.3
45.4
160.0
489.2
Standard deviation
2.4
7.5
22.7
2.4
8.8
20.5
C V (%)
5.26
4.69
4.8
5.29
5.5
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1α in samples. No significant cross-reactivity or interference between Rat IL-1α and analogues was observed.
