Description
Rat IL-12 ELISA Kit
The Rat IL-12 ELISA Kit is a cutting-edge assay meticulously crafted for the quantitative determination of Interleukin-12 (IL-12) levels in diverse rat biological samples. IL-12 is a pivotal cytokine with essential immunological functions, as it orchestrates T cell differentiation, promotes interferon-gamma production, and stimulates the immune response against pathogens.
By measuring IL-12 levels accurately, researchers gain insights into immune regulation, adaptive immunity, and inflammatory processes, which are crucial for investigating various diseases and immune-related conditions. Our ELISA kit ensures exceptional sensitivity and specificity, guaranteeing precise and reproducible results vital for advancing your scientific investigations. Manufactured under strict quality control measures, this kit delivers reliable performance and user-friendly protocols, making it a preferred tool for researchers seeking to deepen their understanding of the immunological landscape. Trust the Rat IL-12 ELISA Kit to empower your research endeavors and unlock the mysteries of immune modulation and inflammatory responses.
| Product Name: | Rat IL-12 ELISA Kit |
| Product Code: | AEES00314 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat IL-12 in samples. No significant cross-reactivity or interference between Rat IL-12 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat IL-12 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-12. You can calculate the concentration of Rat IL-12 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
