The Rat IL-6 ELISA Kit is a specialized assay intended for the precise quantitative determination of Interleukin-6 (IL-6) levels in a variety of rat biological samples. IL-6 is a pivotal cytokine with multifaceted roles in inflammatory responses, immune regulation, and hematopoiesis, making it a fundamental component in several physiological and pathological processes. Accurate measurement of IL-6 is critical for comprehending its functions in mediating inflammation, immune reactions, and homeostasis, thus offering crucial insights for therapeutic strategies targeting cytokine modulation.
Our IL-6 ELISA Kit provides exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible data. Manufactured under rigorous quality control measures, this kit offers robust and consistent performance, coupled with user-friendly protocols, making it an ideal tool for researchers aiming to explore the intricate mechanisms underlying IL-6 activities in various biological contexts.
Product Name:
Rat IL-6 ELISA Kit
SKU:
AEES00297
Target:
Rat IL-6
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-94
95-107
96-109
Average (%)
90
102
101
1:4
Range (%)
93-102
99-108
88-99
Average (%)
96
103
92
1:8
Range (%)
93-103
89-100
87-101
Average (%)
100
97
94
1:16
Range (%)
86-96
101-108
83-93
Average (%)
90
105
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
89-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
34.58
93.2
396.79
34.39
95.73
379.8
Standard deviation
2.04
4.5
19.96
1.75
4.79
18.61
C V (%)
5.9
4.83
5.03
5.09
5.0
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-6 in samples. No significant cross-reactivity or interference between Rat IL-6 and analogues was observed.
