The Rat IL11 CLIA Kit is a highly sensitive assay designed for the precise measurement of Interleukin-11 (IL-11) levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and accuracy, ensuring dependable and consistent results for a variety of research purposes.IL-11 is a key cytokine that plays a crucial role in the regulation of various biological processes, including cell growth, differentiation, and immune response. It is known to be involved in the pathogenesis of several diseases such as inflammation, cancer, and osteoporosis, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Rat IL11 CLIA Kit is a valuable tool for researchers looking to gain insights into the role of IL-11 in health and disease, ultimately paving the way for new discoveries and treatment strategies. Order now and elevate your research with accurate and meaningful data.
Product Name:
Rat IL-11 CLIA Kit
SKU:
RTES00340
Target:
Rat IL-11
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-11. You can calculate the concentration of Rat IL-11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
101-114
93-105
Average (%)
105
107
98
1:4
Range (%)
101-117
103-115
86-101
Average (%)
110
109
92
1:8
Range (%)
86-98
97-111
87-103
Average (%)
93
103
94
1:16
Range (%)
98-114
100-115
98-115
Average (%)
105
106
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.73
72.46
227.97
26.87
77.98
216.1
Standard deviation
2.75
8.68
21.75
2.51
5.75
19.67
C V (%)
9.92
11.98
9.54
9.34
7.37
9.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-11 in samples. No significant cross-reactivity or interference between Rat IL-11 and analogues was observed.
