The Rat IL12 CLIA Kit is specifically designed for the quantitative detection of Interleukin 12 (IL12) levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.IL12 is a critical cytokine involved in regulating the immune response and inflammation, making it an important biomarker in studies related to immune disorders, infectious diseases, and autoimmune conditions.
By detecting IL12 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potential therapeutic strategies.Overall, the Rat IL12 CLIA Kit is a valuable tool for researchers seeking to investigate the role of IL12 in different biological processes and pathological conditions in rat models.
Product Name:
Rat IL-12 CLIA Kit
SKU:
RTES00041
Target:
Rat IL-12
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-12. You can calculate the concentration of Rat IL-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
94-109
91-105
Average (%)
110
99
97
1:4
Range (%)
97-115
96-112
89-103
Average (%)
105
102
96
1:8
Range (%)
97-113
93-108
93-108
Average (%)
103
99
100
1:16
Range (%)
92-104
96-111
93-108
Average (%)
98
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
103
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.1
59.17
182.85
24.3
58.69
168.51
Standard deviation
2.73
5.04
15.03
2.83
6.22
13.13
C V (%)
11.82
8.52
8.22
11.65
10.6
7.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-12 in samples. No significant cross-reactivity or interference between Rat IL-12 and analogues was observed.
