The Rat IL-15 CLIA Kit is a cutting-edge assay designed for the precise measurement of Interleukin-15 (IL-15) levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research purposes.IL-15 is a key cytokine involved in immune responses and inflammation, playing a crucial role in the regulation of T cell function and promoting the proliferation and activation of natural killer cells.
Its dysregulation is associated with various autoimmune disorders and inflammatory diseases, making it a valuable biomarker for investigating these conditions and developing potential treatments.With the Rat IL-15 CLIA Kit, researchers can confidently study the role of IL-15 in rat models, advancing our understanding of immune responses and inflammation mechanisms. Get reliable and reproducible results with this advanced assay kit from Assay Genie.
Product Name:
Rat IL-15 CLIA Kit
SKU:
RTES00342
Target:
Rat IL-15
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-15. You can calculate the concentration of Rat IL-15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
85-99
97-110
Average (%)
93
91
103
1:4
Range (%)
102-118
99-114
91-102
Average (%)
108
105
97
1:8
Range (%)
99-116
99-116
91-104
Average (%)
107
107
98
1:16
Range (%)
101-115
100-118
96-111
Average (%)
108
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.97
78.18
243.82
26.96
74.91
223.64
Standard deviation
2.35
7.39
26.26
2.95
8.91
25.09
C V (%)
9.05
9.45
10.77
10.94
11.89
11.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-15 in samples. No significant cross-reactivity or interference between Rat IL-15 and analogues was observed.
