Based on the information in the provided URL for the Rat IL17 CLIA Kit, we present:The Rat IL17 CLIA Kit is a cutting-edge diagnostic tool designed for the quantitative measurement of Interleukin-17 (IL-17) levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.IL-17 is a key cytokine involved in the regulation of inflammatory responses and immune system function.
Elevated levels of IL-17 have been linked to various autoimmune diseases, including rheumatoid arthritis and multiple sclerosis. The Rat IL17 CLIA Kit provides a valuable tool for studying these conditions and exploring potential therapeutic interventions.Don't miss out on the opportunity to accurately measure IL-17 levels in rat samples with the Rat IL17 CLIA Kit. Order now and take your research to the next level.
Product Name:
Rat IL-17 CLIA Kit
SKU:
RTES00344
Target:
Rat IL-17
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-17. You can calculate the concentration of Rat IL-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
103-116
92-109
Average (%)
97
109
99
1:4
Range (%)
99-115
85-95
96-111
Average (%)
106
90
104
1:8
Range (%)
93-106
85-99
97-112
Average (%)
100
91
104
1:16
Range (%)
88-104
94-111
87-99
Average (%)
95
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
101-118
107
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.36
61.81
200.24
26.88
67.08
201.15
Standard deviation
3.29
6.99
21.99
2.88
7.81
21.12
C V (%)
12.97
11.31
10.98
10.71
11.64
10.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-17 in samples. No significant cross-reactivity or interference between Rat IL-17 and analogues was observed.
