The Rat IL-18 CLIA Kit is specifically designed for the precise measurement of interleukin-18 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results that are perfect for various research applications.Interleukin-18 is a key cytokine involved in the regulation of immune responses and inflammatory processes. It plays a crucial role in various disease conditions, including autoimmune disorders, inflammatory diseases, and infectious diseases.
As a valuable biomarker, studying IL-18 levels can provide important insights into disease mechanisms and potential therapeutic targets.With its advanced technology and reliable performance, the Rat IL-18 CLIA Kit is an essential tool for researchers looking to investigate the role of IL-18 in disease pathology and explore new avenues for treatment development. Trust this kit for robust and dependable results in your research endeavors.
Product Name:
Rat IL-18 CLIA Kit
SKU:
RTES00345
Target:
Rat IL-18
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-18. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-18. You can calculate the concentration of Rat IL-18 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
92-106
101-118
Average (%)
94
98
108
1:4
Range (%)
104-120
99-113
87-103
Average (%)
110
106
94
1:8
Range (%)
91-108
95-111
89-101
Average (%)
99
103
94
1:16
Range (%)
94-110
102-116
103-119
Average (%)
100
107
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.14
78.39
209.51
22.74
83.69
206.53
Standard deviation
2.96
6.41
21.54
2.46
6.25
21.62
C V (%)
12.79
8.18
10.28
10.82
7.47
10.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-18 in samples. No significant cross-reactivity or interference between Rat IL-18 and analogues was observed.
