The Rat IL-1R2 ELISA Kit is a specialized assay designed for the quantitative assessment of Interleukin-1 Receptor Type 2 (IL-1R2) levels in a variety of rat biological samples.
IL-1R2 plays a crucial role in regulating the biological effects of IL-1, a key pro-inflammatory cytokine involved in diverse cellular processes such as immune response, inflammation, and tissue damage. IL-1R2 acts as a decoy receptor, modulating IL-1 signaling and mitigating its pro-inflammatory effects, thereby influencing immune responses and inflammatory cascades. This ELISA kit offers researchers a reliable method to accurately measure IL-1R2 levels, providing essential insights into the intricate regulatory mechanisms of the IL-1 family. By quantifying IL-1R2 expression, researchers can unravel the nuanced interplay between IL-1 and its receptors, shedding light on the complex immune responses and inflammatory pathways orchestrated by IL-1 signaling. Understanding the role of IL-1R2 is crucial for elucidating its impact on disease processes, immune modulation, and therapeutic interventions targeting IL-1 signaling pathways. With its exceptional sensitivity and specificity, the Rat IL-1R2 ELISA Kit delivers precise and reproducible results, enabling researchers to conduct detailed investigations into the regulatory functions of IL-1R2 in immunological contexts. Manufactured under stringent quality standards, this kit ensures robust performance and user-friendly operation, making it an ideal choice for researchers seeking to explore the intricate roles of IL-1R2 in immune regulation and inflammatory responses.
Product Name:
Rat IL-1R2 ELISA Kit
SKU:
AEES00555
Target:
Rat IL-1R2
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1R2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1R2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1R2. You can calculate the concentration of Rat IL-1R2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-107
98-114
Average (%)
97
99
105
1:4
Range (%)
93-107
89-103
94-106
Average (%)
98
95
101
1:8
Range (%)
93-108
90-101
100-113
Average (%)
98
96
106
1:16
Range (%)
90-103
91-106
102-115
Average (%)
96
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
101
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.25
101.76
487.92
47.89
91.68
472.62
Standard deviation
2.83
4.43
18.83
2.47
5.43
19.33
C V (%)
5.64
4.35
3.86
5.15
5.92
4.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-1R2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1R2 in samples. No significant cross-reactivity or interference between Rat IL-1R2 and analogues was observed.
