The Rat IL-21 CLIA Kit is specifically designed to detect interleukin-21 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields.Interleukin-21 is a cytokine that plays a crucial role in regulating immune responses and inflammation. Its dysregulation has been linked to autoimmune diseases, inflammatory disorders, and cancer.
By accurately measuring IL-21 levels, researchers can gain insights into the immune system's functions and potentially develop new therapeutic strategies for these conditions.Overall, the Rat IL-21 CLIA Kit offers a convenient and precise way to study interleukin-21 in rat samples, making it an essential tool for advancing research in immunology and related fields.
Product Name:
Rat IL-21 CLIA Kit
SKU:
RTES00346
Target:
Rat IL-21
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-21. You can calculate the concentration of Rat IL-21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
88-102
103-116
Average (%)
94
93
109
1:4
Range (%)
91-104
86-100
94-107
Average (%)
98
93
101
1:8
Range (%)
99-116
87-100
101-116
Average (%)
107
92
109
1:16
Range (%)
97-111
96-110
97-113
Average (%)
104
101
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
93-106
101
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.45
52.78
248.93
26.44
50.74
257.96
Standard deviation
2.73
5.82
26.66
2.13
5.57
26.72
C V (%)
11.17
11.03
10.71
8.06
10.98
10.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-21 in samples. No significant cross-reactivity or interference between Rat IL-21 and analogues was observed.
