The Rat IL-27 CLIA Kit is specifically designed for the precise measurement of IL-27 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.IL-27 is a key cytokine that plays a critical role in regulating immune responses and inflammation. It has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer.
By accurately measuring IL-27 levels, researchers can gain valuable insights into the role of this cytokine in disease pathogenesis and potential therapeutic interventions.Overall, the Rat IL-27 CLIA Kit is a valuable tool for studying the role of IL-27 in various biological processes and pathological conditions, offering researchers a reliable and efficient method for quantifying IL-27 levels in rat samples.
Product Name:
Rat IL-27 CLIA Kit
SKU:
RTES00348
Target:
Rat IL-27
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-27. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-27, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-27. You can calculate the concentration of Rat IL-27 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
94-105
96-108
Average (%)
100
99
103
1:4
Range (%)
97-110
90-104
97-110
Average (%)
104
95
104
1:8
Range (%)
95-111
91-107
100-117
Average (%)
103
99
108
1:16
Range (%)
91-105
88-102
86-96
Average (%)
96
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.7
66.2
210.75
30.06
63.42
191.56
Standard deviation
2.92
6.04
17.3
3.8
6.37
15.25
C V (%)
10.54
9.12
8.21
12.64
10.04
7.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-27 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-27 in samples. No significant cross-reactivity or interference between Rat IL-27 and analogues was observed.
