The Rat IL-5 CLIA Kit is specifically designed for the quantitative measurement of Interleukin-5 (IL-5) levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, allowing for precise and reliable results in various research applications. IL-5 is a key cytokine involved in promoting the growth and differentiation of eosinophils, a type of white blood cell that plays a crucial role in inflammatory responses and allergic reactions.
Abnormal levels of IL-5 have been linked to conditions such as asthma, allergic rhinitis, and autoimmune diseases, highlighting the importance of monitoring IL-5 levels in experimental studies. By using the Rat IL-5 CLIA Kit, researchers can gain valuable insights into the role of IL-5 in immune responses, inflammation, and disease pathology, ultimately contributing to the development of novel therapeutic strategies.
Product Name:
Rat IL-5 CLIA Kit
SKU:
RTES00338
Target:
Rat IL-5
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-5. You can calculate the concentration of Rat IL-5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
95-108
95-108
Average (%)
96
102
101
1:4
Range (%)
91-105
88-99
89-105
Average (%)
99
93
96
1:8
Range (%)
87-101
91-104
86-96
Average (%)
93
96
91
1:16
Range (%)
90-105
95-107
92-105
Average (%)
96
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.73
41.74
170.21
26.84
44.87
162.07
Standard deviation
2.55
3.16
17.87
2.47
3.79
11.81
C V (%)
10.31
7.57
10.5
9.2
8.45
7.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-5 in samples. No significant cross-reactivity or interference between Rat IL-5 and analogues was observed.
