The Rat IL-7 CLIA Kit is specifically designed for the quantitative measurement of Interleukin-7 (IL-7) levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in various research applications.IL-7 is a pivotal cytokine that plays a crucial role in immune system development and function. It is involved in the maturation and proliferation of T cells, making it essential for maintaining a healthy immune response.
Dysregulation of IL-7 levels has been linked to various immunological disorders and autoimmune diseases, underscoring the importance of studying this biomarker.With the Rat IL-7 CLIA Kit, researchers can confidently analyze IL-7 levels in rat samples, providing valuable insights into immune system function and potential therapeutic targets. This kit is a valuable tool for enhancing research in immunology, inflammation, and autoimmune diseases.
Product Name:
Rat IL-7 CLIA Kit
SKU:
RTES00339
Target:
Rat IL-7
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-7. You can calculate the concentration of Rat IL-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
99-111
103-114
Average (%)
99
105
108
1:4
Range (%)
90-105
88-101
87-100
Average (%)
97
93
92
1:8
Range (%)
100-117
92-104
84-97
Average (%)
108
99
90
1:16
Range (%)
97-109
90-106
86-98
Average (%)
102
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
88-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.08
55.74
204.55
23.43
59.23
190.94
Standard deviation
2.93
4.25
14.01
2.32
4.95
11.7
C V (%)
12.17
7.62
6.85
9.9
8.36
6.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-7 in samples. No significant cross-reactivity or interference between Rat IL-7 and analogues was observed.
