Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
The Rat IP-10 (CXCL10) Interferon-Gamma Induced Protein 10kDa CLIA Kit (RTES00329) is a powerful tool for detecting levels of IP-10 in rat samples. This kit is designed with high sensitivity and specificity to ensure accurate and reproducible results in serum, plasma, and cell culture supernatants.IP-10, also known as CXCL10, is a key chemokine involved in inflammatory responses and immune regulation. It is induced by interferon-gamma and plays a role in various diseases, including autoimmune diseases, viral infections, and cancer.
By accurately measuring IP-10 levels, researchers can gain valuable insights into the immune response and potential therapeutic interventions.Whether studying inflammation, immune response, or disease pathology, the Rat IP-10 (CXCL10) CLIA Kit is an essential tool for researchers seeking to understand the role of IP-10 in rat models. Order yours today from Assay Genie and unlock the potential of your research.
Product Name:
Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
SKU:
RTES00329
Target:
Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IP-10/CXCL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IP-10/CXCL10. You can calculate the concentration of Rat IP-10/CXCL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
96-108
103-118
Average (%)
93
102
109
1:4
Range (%)
97-111
101-118
90-102
Average (%)
103
109
95
1:8
Range (%)
98-114
91-104
94-108
Average (%)
105
97
102
1:16
Range (%)
83-96
97-109
99-117
Average (%)
90
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
99-110
104
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.44
92.19
284.16
35.54
94.57
267.75
Standard deviation
4.22
10.29
23.05
3.89
7.33
24.5
C V (%)
10.7
11.16
8.11
10.95
7.75
9.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Rat IP-10/CXCL10 and analogues was observed.
